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National Repository of Grey Literature

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	A metodical approach for sanitation of grapevine cultivars using chemotherapy Komínek, Petr ; Jandová, Barbora ; Komínková, Marcela ; Polák, Jaroslav The guideline describe a method of grapevine cultivation in vitro and its sanitation from virus pathogens usinf Ribavirin. Large part of this guideline describe diagnostic protocols for estimation of healthy status of sanitated grapevines. The guideline is intended for laboratories dealing with sanitation and diagnostics of viral pathogens of grapevine cultivars and rootstocks. Further utilization can be done by the Ministry of Agriculture, Central Institute for Supervising and Testing in Agriculture and also by growers of grapevine. Fulltext: PDF Detailed record
	Methods of detection of viruses from cereals and grasses using SYBR Green I RT-qPCR Jarošová, Jana ; Dráb, Tomáš ; Beoni, Eva ; Kumar, Jiban The family Poaceae is one of the most important groups of plants in the terms of agricultural production. The family Poaceae also hosts many pathogens including >100 known virus species. The distribution of the viruses varies according to hosts, geographical and other conditions. In this methodology we provide assays for detection of nine cereal and grass viruses (Brome mosaic virus-BMV, Barley yellow dwarf virus-BYDV, Lolium latent virus-LoLV, Oat necrotic mottle virus-ONMV, Ryegrass mosaic virus-Rg- MV, Soil-borne cereal mosaic virus-SBCMV, Spartina mottle virus-SpMV, Wheat dwarf virus-WDV a Wheat streak mosaic virus-WSMV) by SYBR Green I RT-qPCR. Fulltext: PDF Detailed record
	PCR based assay for detection garden pea lec gene Vráblík, Aleš ; Hodek, Jan ; Ovesná, Jaroslava The method was developed and verified by staff of National reference laboratory for GMO identification and DNA fingerprinting suited for governmental control laboratories and private laboratories that analyze food and feed derived from various plant matrices. Method can be applied for quantification of GM pea in a sample, when reference gene is used as a standard. The method describe internal garden pea specific gene, lektin in this case, detection using PCR and real-time PCR. The general principle of the assay relies on lektin specific sequence presence that represents a species specific gene of garden pea. In many matrices DNA is damaged so amplification of short stretches of DNA is used. After amplification of target exploiting newly developer species specific gene primers PCR products are separated by electrophoresis in agarose gel. Resulting band is visualized by UV light and its position is compare with size standard. Alternatively, real-time PCR and ABI platform in combination with SYBR® Green can be used. Reaction parameters are described and specificity of reaction was verified. LOD as well as LOQ was defined. Fulltext: PDF Detailed record
	Method of identification of Fusarium species causing head blight by PCR Sumíková, Taťána ; Gabrielová-Slezáková, Ludmila ; Žabka, Martin 1. Multiplex PCR reaction with reliable published primers, enabling an easy detection of selected Fusarium species in one reaction, without time consuming optimalization. 2. Method of micromycetes cultivation using medium covered with cellophane, that is very suitable for following DNA extraction (easier harvesting of mycelia, minimal risk of medium contamination). Fulltext: PDF Detailed record
	Methods of detection of wheat and barley strain of Wheat dwarf virus in vector Psammotettix alienus by PCR-RFLP Jaňourová, Blanka ; Ripl, Jan ; Kumar, Jiban Wheat dwarf (causal agent Wheat Dwarf Virus - WDV) is one of the most important cereal diseases in the Czech republic where it can cause huge economic losses in wheat, barley, triticale, rye as well as in oat. Psammotettix alienus is the only known vector of WDV while another way of transmission is unrecognized. This methodology describes a PCR assay for sensitive detection of Wheat Dwarf Virus in Psammotettix alienus, and RFLP assay for barley and wheat strains specific identification. Fulltext: PDF Detailed record
	Methodology of detection Penicillium expansum, Monilia frutigena, Botrytis cinerea and Neofabraea alba using multiplex PCR Sumíková, Taťána ; Gabrielová, Ludmila ; Žabka, Martin Multiplex PCR for rapid and reliable detection of species causing rotting apples: Penicillium expansum, Monilia fructigena, Botrytis cinerea and Neofabraea alba is described here. Reaction is optimized for using in a common molecular laboratory. Described method allows both detectino of the pathogens from pure mycological cultrures, and their detection directly from the rot apple tissues. Cultivation of the micromycethes on cellophane surface is also described. References for mycological determination literature are listed, and can be helpful for determination in case of negative results of PCR. Fulltext: PDF Detailed record
	A metodical approach to detect viruses belonging to a genus Vitivirus infecting grapevines in the Czech Republic Komínek, Petr A metodical approach describes detailed procedure to detect two important grapevine viruses belonging to a genus Vitivirus. Two independent methods are recommended for the detection: RT-PCR and DAS-ELISA. Fulltext: PDF Detailed record
	A metodical approach to evaluate distribution of grapevine viruses in vineyards of the Czech Republic Komínek, Petr A metodical approach describes a procedure focused to evaluate distribution of eight important grapevine viruses using detection based on ELISA method. Fulltext: PDF Detailed record
	Psychologické dotazníky a detekce psychosomatických stavů Zavadil, Petr Detailed record
	Collision States Detection For Quadruped Robot Motion Chmelíček, J. ; Grepl, Robert ; Bezdíček, M. ; Švehlák, M. Detection of collision states among single parts of robot strucure is essential problem, that is necessary to solve at robot movement in virtual space, in mathematical models based on substitutes of real structures by rigid bodies and above all at robot safe movement. Detailed record

National Repository of Grey Literature : 11 records found   1 - 10  jump to record:

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